Expression of MMP-9 and TIMP-1 in the Nasal Mucosa of Allergic Rhinitis.
- Author:
Heung Man LEE
1
;
Jin Ho CHOI
;
Choong Sik CHOI
;
Soon Jae HWANG
;
Sang Hag LEE
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Allergic rhinitis;
MMP-9;
TIMP-1
- MeSH:
Biopsy;
Eosinophils;
Epithelial Cells;
Extracellular Matrix;
Humans;
Image Processing, Computer-Assisted;
Inflammation;
Matrix Metalloproteinases;
Nasal Mucosa*;
Rhinitis*;
Tissue Inhibitor of Metalloproteinase-1*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2000;43(6):604-609
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Allergic rhinitis is characterized by airway structural changes, including inflammation and basement thickening. An imbalance between matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) is thought to play a critical role in the synthesis or degradation of the extracellular matrix of the airway architecture. To elucidate the mechanism of remodeling in allergic rhinitis, we investigated the expressions of MMP-9 and TIMP-1 in the nasal mucosa of allergic rhinitis patients and the control subjects. We also compared the number of infiltrating inflammatory cells (eosinophils) and the immunostained areas of MMP-9 and TIMP-1. MATERIALS AND METHODS: Nasal biopsy specimens were obtained from 20 subjects with allergic rhinitis and 20 healthy control subjects, and the extent of staining by MMP-9 and TIMP-1 was estimated by using immunohistochemicd staining. Using a computer assisted image analysis system, we quantified the expressions of MMP-9 and TIMP-1, and the number of inflammatory cells was also determined. RESULTS: Expressions of MMP-9 and TIMP-1 were observed in the nasal mucosa of epithelial cells and infiltrating inflammatory cells. The extent of immunostained areas of MMP-9 was signi6cantly greater than that of TIMP-1 in the allergic rhinitis patients. A significant correlation was found between the number of eosinophils and the extent of MMP-9 immunostained area. CONCLUSION: The increased expression of MMP-9 in the allergic nasal mucosa suggests that MMP-9 secreted from the infiltrating inflammatory cells and the nasal epithelium causes tissue remodeling in the allergic nasal mucosa.
