Chromosomal Mapping and Brain Distribution of alpha1 Subunit of N-type Voltage Dependent Calcium Channel.
- Author:
Sun Hwa PARK
;
Hyun KIM
;
Hyung Rae KIM
;
Soo Yun PARK
;
Im Joo YOO
;
Young Suk SEO
- Publication Type:Original Article
- Keywords:
N;
Fluorescent in situ hubridzation;
in situ hybridzation N-type voltage dependent calcium channel;
Human genom;
Nervous tissue;
in situ hybridization histochemistry
- MeSH:
Animals;
Arm;
Brain*;
Calcium Channels*;
Calcium Channels, N-Type;
Calcium*;
Cerebellum;
Cerebral Cortex;
Dentate Gyrus;
Gene Expression;
Hippocampus;
Humans;
In Situ Hybridization;
In Situ Hybridization, Fluorescence;
Nervous System;
Neurites;
Neurons;
Neurotransmitter Agents;
Olfactory Bulb;
Rats;
RNA, Messenger
- From:Korean Journal of Anatomy
1997;30(4):343-350
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Voltage dependent calcium channels mediate wide variety of physiological functions including neurotransmitter release, neurite outgrowth, and gene expression in neurons. omega-Conotoxin-sensitive N-type calcium channels are exclusively expressed in nervous system and involved in the control of neurotransmitter release from neurons. In this experiment, I have investigated human chromosomal location and rat neuronal distribution of N-type voltage dependent calcium channel alpha1, subunit [alpha1B]. I have localized human alpha1B subunit gene to the long arm of chromosome 9[9q34] by fluorescent in situ hybridization. The distribution of rat alphaB1 subunit mRNA has been examined in the rat brain by in situ hybridization histochemistry and high level of alpha1B subunit mRNA has been observed in olfactory bulb, anterior olfactory nucleus, cerebral cortex, piriform cortex, hippocampus, dentate gyrus, parabrachial nucleus, and cerebellum and low level of expression was also found in other areas of rat brain.
