Histologic Similarity between the Primary Tumor and Xenograft of AMC-HN Cell Lines in Nude Mice.
- Author:
Hyung Kyung JUN
1
;
Sang Yoon KIM
;
Gyung Yub GONG
Author Information
1. Department of Otorhinolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Squamous cell carcinoma cell line;
Nude mouse;
Histology
- MeSH:
Animals;
Biology;
Carcinoma;
Cell Line*;
Cell Line, Tumor;
Chungcheongnam-do;
Head;
Heterografts*;
Humans;
Male;
Mice;
Mice, Nude*;
Neck;
Ulsan
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1997;40(11):1546-1553
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Head and neck cancer(HNC) is the sixth most common cancer worldwide. Squamous cell carcinoma(SCC) accounts for 85-90% of all HNC. However, its biologic behavior has little been known yet. OBJECTIVES: In the present investigation, Eight of nine cell lines, which were developed and characterized in Asan Medical Center of University of Ulsan, were tested for tumor formation and histology in nude mice. MATERIALS AND METHODS: Explant cultures of fresh tumor tissue were used to develop new permanent tumor cell lines. Five-to ten million-cells were injected subcutaneously into 3-to 6-week-old male nude mice and the formation of tumors was evaluated after 3 to 8 weeks. RESULTS: Eight cell lines(AMC-HN-1, -2, -3, -4, -5, -6, -7 and -9) produced progressively growing tumor, and were noticed remarkable similarity of the histologic feature to original tumor. Well formed keratin pearls were present both in the original tumor and the tumor produced by the AMC-HN-3 and -7 cell lines. AMC-HN-2 and -6 grew in well formed infiltrating cords with a prominent stromal network exactly like that in the original tumor. The undifferentiated carcinoma from AMC-HN-9 has the same histologic and immunohistochemical pattern. CONCLUSION: The histologic similarity between original tumors and AMC-HN cell lines are representative that cell lines are promised to be good in vitro models for study of HNC biology.
