Immunocytochemical Expression of Amylin in Pancreatic Islets of Man, Rabbit and Guinea Pig.
- Author:
Sung Gyung KIM
;
Young Hee KIM
;
Jung Ho CHA
;
Jin KIM
;
Jin Woong JUNG
- Publication Type:Original Article
- Keywords:
Pancreatic islet;
Immunocytochemistry;
Man;
Rabbit;
Guinea pig
- MeSH:
Amyloid;
Animals;
B-Lymphocytes;
Glucagon;
Guinea Pigs*;
Guinea*;
Immune Sera;
Immunohistochemistry;
Insulin;
Islet Amyloid Polypeptide*;
Islets of Langerhans*;
Rats;
Somatostatin-Secreting Cells
- From:Korean Journal of Anatomy
1997;30(4):411-420
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
A putative polypeptide hormone identified as amylin[islet amyloid polypeptide] is synthesized and co-localized with insulin in B cells of pancreatic islets in several animal species including man. However, there is growing evidence that somatostatin cells are also expressed and contained amylin in the pancreatic islets of the rat The aim of the present study was to investigate the immunocytochemical expression of the amylin within the endocrine pancreas of the man, rabbit and guinea pig, with special reference to the possible ability of islet cells other than insulin cells to synthesize amylin. For this purpose serial sections of the pancreatic islets were stainedimmunocytochemically using anti-amylin, anti-insulin, anti-glucagon, anti-somatostatin antisera. In serial sections of pancreatic islets of the man and rabbit, it was shown that amylin immunoreactivity occurred in insulin-reactive B cells predominantly located in interior of the islets. In contrast, amylin immunoreacivity appeared in glucagon-reactive A cells peripherally located in the islets of the guinea pig. These results suggest that in both the man and rabbit, amylin is synthesized by B cells for subsequent co-secretion with insulin, and that in guinea pig, amylin is synthesized by A cells for co-secretion with glucagon. It thus appears that amylin release may be mediated by different secretory mechanisms according to animal species.
