Effects of Blood-Brain Barrier Disruption on Cerebral Oxygen Balance.
- Author:
Doo Ik LEE
1
;
Young Kyu CHOI
;
Dong Ok KIM
;
Keon Sik KIM
;
Ok Young SHIN
;
Moo Il KWON
Author Information
1. Department of Anesthesiology, Kyung Hee University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Brain;
blood-brain barrier;
cerebral oxygen balance;
Rats
- MeSH:
Animals;
Blood-Brain Barrier*;
Brain;
Carotid Artery, Internal;
Catecholamines;
Excitatory Amino Acids;
Isoflurane;
Mannitol;
Microspectrophotometry;
Neurotransmitter Agents;
Oxygen*;
Permeability;
Rats;
Respiration, Artificial;
Tracheostomy;
Vital Signs
- From:The Korean Journal of Critical Care Medicine
1999;14(2):126-130
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGOUND: Disruption of the blood-brain barrier (BBB) can alter the internal milieu and may increase the release of excitatory amino acid neurotransmitters or catecholamines, which may affect metabolic rate or coupling. This study was performed to evaluate whether disruption of BBB by unilateral intracarotid injection of hyperosmolar mannitol would alter oxygen supply/consumption balance in the ipsilateral cortex. METHODS: Rats were anesthetized with 1.4% isoflurane using mechanical ventilation via tracheostomy. 25% mannitol was administered at a rate of 0.25 mlxkg-1s-1 for 30 s through unilateral internal carotid artery. The BBB transfer coefficient (Ki) of 14C-alpha-aminoisobutyric acid was measured in one group (N=7) after administering mannitol. Regional cerebral blood flow (rCBF), regional arterial and venous O2 saturation and O2 consumption were measured in another group using a 14C-iodoantipyrine and microspectrophotometry (N=7). RESULTS: Vital signs were similar before and after administering mannitol. Ki was significantly higher in the ipsilateral cortex (IC) than in the contralateral cortex (CC), (22.3+/-8.4 vs 4.4+/-1.1 microliterxg-1min-1). rCBF was similar between IC (105+/-21 mlxg-1min-1) and the CC (93+/-20). Venous O2 saturation was lower in the IC (43+/-7%) than in the CC (55+/-4). O2 consumption was higher in the IC (9.6+/-3.0 mlx100 g-1min-1) than in the CC (6.7+/-1.5). CONCLUSIONS: Our data suggested that increasing permeability of the BBB increased cerebral O2 consumption and deteriorated cerebral oxygen balance.
