Optimization of Recombinant Human Platelet-Derived Growth Factor-BB Encapsulated in Poly (Lactic-co-Glycolic Acid) Microspheres for Applications in Wound Healing.
10.1007/s13770-015-0029-z
- Author:
Yun Ju BAE
1
;
Chi Heung CHO
;
Woo Jong LEE
;
Jeung Soo HUH
;
Jeong Ok LIM
Author Information
1. Department of Biomedical Science, Graduate School, Kyungpook National University, Daegu, Korea. jolim@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Recombinant human platelet-derived growth factor-BB;
Poly (lactic-co-glycolic acid) microsphere;
Wound healing;
Human immortalized keratinocyte cell line;
Migration
- MeSH:
Half-Life;
Humans*;
In Vitro Techniques;
Intercellular Signaling Peptides and Proteins;
Methods;
Microspheres*;
Platelet-Derived Growth Factor;
Polymers;
Wound Healing*;
Wounds and Injuries*
- From:
Tissue Engineering and Regenerative Medicine
2016;13(1):13-20
- CountryRepublic of Korea
- Language:English
-
Abstract:
Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An in vitro migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.
