Mouse fibroblast growth factors-21 (MFGF-21) isolation
- Author:
Nguyen Bich Nhi
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor
- MeSH:
Fibroblast Growth Factors;
Mice;
Liver
- From:Journal of Practical Medicine
2003;442(2):109-111
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Adult made IRC white mouse of 21 week age, weighing 22-25 g underwent an operation for removing the liver, which was washed with PBS buffer. Total ARN extracted from clean mouse liver composes of 3 characteristic band 28S, 18S and 5S. Total ARN concentration received was 10Ug/Ul approximatively. Received PCR product is a characteristic band of the size of some 630bp corresponded to the size of mFGF-21. It is a total gene of mFGF-21 with a sequence 100% corresponding to the registered sequence in the gene international data Bank Number NM-020013
