Quantitative Autoradiography on Ontogenic Development of alpha-Adrenoceptor in the Rat Cerebral Cortex.
- Author:
Myoung Kon LEE
1
;
Seung Won LEE
;
Ji Hyun KOOK
;
Yoon Young CHUNG
;
Eun Joo LEE
;
Jong Keun KIM
;
Choon Sang BAE
Author Information
1. Department of Anatomy, Chonnam University Medical School, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
alpha- adrenergic receptor;
Rat cerebral cortex;
ontogeny;
in vitro autoradiography;
binding density
- MeSH:
Adult;
Animals;
Autoradiography*;
Binding Sites;
Catecholamines;
Cerebral Cortex*;
Humans;
Parturition;
Phentolamine;
Rabeprazole;
Rats*;
Receptors, Adrenergic;
Yohimbine
- From:Korean Journal of Anatomy
1999;32(5):749-760
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Adrenoceptors mediate response to catecholamines throughout the body. To investigate postnatal ontogenic development of alpha1- and alpha2- adrenoceptors in the rat cerebral cortex, in vitro autoradiography was done on frontal, parietal and temporal cortex in P0, P5, P10, P15, P20, P30 and adult animals. Binding sites for the alpha1-adrenergic receptor ligand, [3H]-prazosin, and the alpha2-adrenergic receptor ligand, [3H]-rauwolscine, were visualized by in vitro autoradiography, and anatomically localized by comparing the autoradiogram to Nissl-stained sections. Nonspecific binding was detected with unlabeled phentolamine (alpha1) and yohimbine (alpha2). There is uniform increase in alpha1- and alpha2- adrenoceptors from birth through first three or four postnatal weeks, followed by a decrease to adult level. Two alpha-adrenoceptors have very different ontogenic patterns of distribution during postnatal development. alpha1- adrenoceptors were expressed differentially in different cortical (frontal, temporal, parietal) regions and in different cortical layers (layers V, II-IV, VI) at same age. alpha2- adrenoceptor was expressed homogenously in throughout regions and layers. These findings may provide evidence that alpha1- adrenoceptors are involved in regulating cortical development or function more specifically than alpha2- adrenoceptors during postnatal development.
