The Efficient Gene Delivery into Human Mesenchymal Stem Cells Using Retroviral Vectors.
- Author:
Sung Soo KIM
1
;
Bum Jun KIM
;
Haeyoung Suh KIM
Author Information
1. Department of Anatomy, School of Medicine, Ajou University, 442-749, Suwon, Korea. hysuh@ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Gene therapy;
Retrovirus;
Transfection
- MeSH:
Autografts;
Genetic Therapy;
Humans*;
Indicators and Reagents;
Mesenchymal Stromal Cells*;
Multipotent Stem Cells;
Retroviridae;
Transfection;
Transplantation, Autologous;
Zidovudine*
- From:Korean Journal of Anatomy
2003;36(5):381-387
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human mesenchymal stem cells (hMSCs) are multipotent stem cells that can differentiate into several mesenchymal lineage cells. In this study, we established an efficient method for gene delivery into these cells. Non-viral transfection reagents that were commercially available yielded 5% efficiency. In contrast, a retroviral vector yielded more than 46% transduction, which was further increased to 90% by repetitive infection. Retroviral transduction did not alter the multipotency of hMSCs. Thus, the cells retained the potential to differentiate into adipogenic, chondrogenic, or osteogenic lineages. The conditions established in this study will contribute to development of trans-differentiation methods of hMSCs into non-mesodermal lineage cells and thereby facilitate their possible use as vehicles for autologous transplantation in both cell and gene therapy for various diseases.
