In vivo Study on the Japanese Encephalitis: Viral Localization and Histopathology in the Mouse Brain.
- Author:
In Beom KIM
1
;
Soo Lim CHAE
;
Woo Young CHOI
;
Chan PARK
;
Young Ran JOO
;
Hae Wol CHO
;
Keun Yong PARK
Author Information
1. Department of Virology, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, Korea. ibkimmd@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Japanese encephalitis virus;
Immunohistochemistry;
Histopathology;
In vivo model;
Mouse
- MeSH:
Animals;
Asian Continental Ancestry Group*;
Brain*;
Central Nervous System;
Encephalitis;
Encephalitis Virus, Japanese;
Encephalitis, Japanese*;
Encephalitis, Viral;
Far East;
Humans;
Immunohistochemistry;
Mice*;
Neurons;
Tropism;
Virus Internalization;
Young Adult
- From:Korean Journal of Anatomy
2003;36(5):427-433
- CountryRepublic of Korea
- Language:English
-
Abstract:
Japanese encephalitis is a potentially lethal disease of the central nervous system caused by infection with Japanese encephalitis virus (JEV). JEV is the most common cause of encephalitis over a large part of eastern Asia. To establish and characterize in vivo model to study the Japanese encephalitis, the immunohistochemical localization of JEV and the histopathological finding were investigated in the brains of young adult mice infected with JEV by intraperitoneal inoculation. JEV was localized to neurons in discrete regions of the brain. Histopathological finding showed typical pattern of acute viral encephalitis, such as inflammatory cell infiltration in brain parenchyme and perivascular cuffs of mononuclear cells. These results suggest that this in vivo system can be used to study the mechanism of virus entry into the brain, cell specific tropism, and pathophysiology in Japanese encephalitis.
