The Effect of a High Dose of Methotrexate on Type II Collagen induced Arthritis in Rats.
10.4055/jkoa.2002.37.1.123
- Author:
Jung Hwan SON
1
;
Tae Hyuk SUH
;
Gu Hee JUNG
;
Jae Do KIM
Author Information
1. Department of Orthopaedic Surgery, Kosin University Gospel Hospital, Busan, Korea. jhson@ns.kosinmed.or.kr
- Publication Type:Original Article
- Keywords:
Brown Norway rat;
High-dose methotrexate;
Type II collagen- induced arthritis
- MeSH:
Animals;
Arthritis*;
Arthritis, Rheumatoid;
Cell Count;
Collagen;
Collagen Type II*;
Humans;
Injections, Intraperitoneal;
Male;
Methotrexate*;
Norway;
Rats*
- From:The Journal of the Korean Orthopaedic Association
2002;37(1):123-130
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Rat arthritis was induced by bovine type II collagen. To present the remedial effects on type II collagen arthritis and clinical efficacy, a high dose of methotrexate, of a level similar to that used in cancer patients was given to rats. MATERIALS AND METHODS: Healthy male Brown Norway rats were used, of mean weight; 300 g. There were 7 subgroups: a control group, given collagen only, an orally methotrexate treated (2.5mg/week), high methotrexate dose groups; 10 mg/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg and a low dose methotrexate group (0.375 mg/kg/week, 4 times) were included in the present study. Type II collagen was injected for arthritis induction and a single intraperitoneal injection of methotrexate was administered after 15 days. Orally treated group was administered 4 times (2.5 mg/week). Histopathologic findings were evaluated. RESULTS: 50 mg/kg methotrexate was effectively reduced the arthritis index and significantly decreased inflammatory cell number between 2nd and 6 weeks. CONCLUSION: A high dose of methotrexate may be clinically effective in the short period treatment of rheumatoid arthritis patients.
