Proliferation of Mature T Cells in the Periphery of the Mice.
- Author:
Dong Sup LEE
1
;
Wang Jae LEE
Author Information
1. Department of Anatomy, Seoul National University, College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Mouse;
CD4 T cell;
CD8 T cell
- MeSH:
Adult;
Animals;
Concanavalin A;
Humans;
Lymph Nodes;
Lymphocytes;
Mice*;
Phenotype;
T-Lymphocytes*;
Tissue Donors
- From:Korean Journal of Anatomy
1999;32(5):717-726
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to find out the proliferative ability and functional capacity of peripherally expanded mature T cells, syngeneic lymph node cells were injected into thymectomized and irradiated adult mice. Donor and host T cells were evaluated in specific time points. The results were as followings. 1. As compared to the engrafted lymphocytes after transfer, we observed 54~60 fold increase in CD4 T cells and 113~130 fold increase in CD8 cells, that is, CD8 cells proliferated more than CD4 cells in the periphery. 2. Mature T cells proliferated in the periphery got activated/memory phenotype in the early period of expansion but they upregulated CD45RB and CD62L in the later period. CD44 level remained high. 3. T cells maintained their response against soluble antigen, KLH but the reactivity against some polyclonal T cell activators, such as anti-CD3 antibody and concanavalin A were reduced severely in the 6 month expanded group. 4. The leftover host T cells also proliferated in the space and they behave similar to donor T cells in proliferation and change of activation/memory markers.
