The Influence of Fibronectin and/or RGDS Tetrapeptide on Osteopontin Expression in Cultures of Rat Calvarial Osteoblasts.
10.4055/jkoa.2002.37.1.145
- Author:
Suk Ku HAN
1
;
Hyoung Min KIM
;
Nam Yong CHOI
;
Ho Gun KIM
;
Jae Do HA
Author Information
1. Department of Orthopedic Surgery, St. Paul's Hospital, Korea.
- Publication Type:Original Article
- Keywords:
Osteoblast;
Fibronectin;
RGDS synthetic tetrapeptide;
AW-GC;
Osteopontin
- MeSH:
Alkaline Phosphatase;
Animals;
Blotting, Western;
Cell Proliferation;
Ceramics;
Fibronectins*;
Glass;
Osteoblasts*;
Osteopontin*;
Peptides;
Polystyrenes;
Rats*;
RNA, Messenger
- From:The Journal of the Korean Orthopaedic Association
2002;37(1):145-151
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of fibronectin (FN) and/or RGDS tetrapeptide on osteoblastic proliferation and osteopontin expression. MATERIALS AND METHODS: Osteoblasts were cultured on tissue culture polystyrene (TCPS) and apatite-wallastonite glass ceramics (AW-GC) with or without FN and RGDS synthetic tetrapeptide. Osteoblastic proliferation and differentiation were measured after cultivation, by determining the number of attached cells, by [3H]-thymidine assay, and by measuring the activity of alkaline phosphatase. The expression of osteopontin (OPN) was determined by RT-PCR and western blotting. RESULTS: Cellular proliferation was more increased by FN than the other variables examined (P<0.03). OPN mRNA expression by RT-PCR was induced two-fold, 24-hour after FN treatment (P<0.05). The expression of OPN by western blotting showed a five-fold increase in cells treated with FN compared with the controls. The synthetic RGDS peptides partially blocked the growth of cells induced by FN (P<0.05). RGDS tetrapeptide alone increased the OPN expression induced by cultured osteoblasts (P<0.05). CONCLUSION: FN enhanced osteoblastic proliferation on glass ceramics and induced OPN expression. Integrin occupancy by RGD-containing molecules may play a role in the process of osteoblastic proliferation rather than in osteoblastic differentiation.
