Agonistforthe Control of Apotosis through the Study of Cytokine Expression after Spinal CordInjuryin Rats.
10.4055/jkoa.2007.42.1.106
- Author:
Jun Young YANG
1
;
K Daniel RIEW
Author Information
1. Department of Orthopaedic Surgery, School of Medicine, Chungnam National University, Research Institute of Medical Sciences, Daejeon, Korea. jyyang@cnuh.co.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Spinal cord injury
- MeSH:
Animals;
Apoptosis;
Cytokines;
Erythropoietin;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor;
Humans;
Laminectomy;
Methylprednisolone;
Rats*;
Rats, Sprague-Dawley;
Riluzole;
Sodium Channel Blockers;
Spinal Cord Injuries;
Spine;
Steroids
- From:The Journal of the Korean Orthopaedic Association
2007;42(1):106-114
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Purpose: To analyze the cytokines that appear after a spinal cord injury in rats and to determine the agonists that regulate apoptosis. Materials and Methods: Sixty female Sprague-Dawley rats were anesthetized, and a laminectomy was performed at the 9th thoracic vertebra. The spinal cord injury was induced by dropping a 10 gm weight at a height of 20 mm. The subjects were divided into 5 groups. Group I was administered aminoguanidine, group II was administered GM-CSF, group III was administered riluzole, group IV was administered erythropoietin, and group V was administered methylprednisolone. A control group was administered normal saline. The results were assessed using the Tarlov motor grading method. In 1, 3, 5 and 7 days after the spinal cord injury, the rats were sacrificed and evaluated using the syringomyelic cavity size. Immunohistochemical staining for e-NOS and RT-PCR for XIAP were also performed. Results: Groups I, III, and V showed significantly different results in terms of the motor recovery and inhibition of increase in the syringomyelic cavity compared with the other groups (p<0.05). The e-NOS activity was observed in groups I, III, and V. The other groups showed almost no e-NOS activity. On the RT-PCR, groups I, III, and V showed significantly different results in terms of XIAP expression with time compared with the other groups. Conclusion: Steroids, NOS inhibitors and sodium channel inhibitors appear to be important factors for regulating apoptosis in the early stage of a spinal cord injury. Further study will be needed to develop new pharmaceuticals with synergic effects on spinal cord injuries.
