Effect of Cysteamine on Human Peripheral Blood Mononuclear Cells-Chemically Injured Keratocytes Reaction.
10.3341/jkos.2015.56.10.1511
- Author:
Young Bok LEE
1
;
Joon Young HYON
;
Won Ryang WEE
;
Tae Young CHUNG
;
Eui Sang CHUNG
;
Ka Young YI
;
Young Joo SHIN
Author Information
1. Department of Ophthalmology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. schinn@hanmail.net
- Publication Type:Original Article
- Keywords:
Cysteamine;
Macrophage migration inhibitory factor;
Mixed peripheral lymphocyte-keratocyte reaction;
Reactive oxygen species;
Transforming growth factor-beta1
- MeSH:
Bromodeoxyuridine;
Burns;
Cysteamine*;
Enzyme-Linked Immunosorbent Assay;
Fluorescence;
Humans*;
Interleukin-6;
Macrophages;
Reactive Oxygen Species;
Transforming Growth Factor beta1
- From:Journal of the Korean Ophthalmological Society
2015;56(10):1511-1519
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of cysteamine on mixed peripheral blood mononuclear cells (PBMCs)-chemically injured keratocytes reaction (mixed lymphocyte-keratocyte reaction; MLKR). METHODS: PBMC stimulation assay was performed after keratocytes were chemically injured with 0.05 N NaOH for 60 seconds. MLKR was treated with various concentrations of cysteamine (0-10 mM). Intracellular reactive oxygen species (ROS) formation was measured using the oxidation-sensitive fluorescent probe, 2'7'-dichlorofluorescein diacetate (DCF-DA). Proliferation rate of PBMCs stimulated by NaOH-treated keratocytes and secretion profiles of matrix metalloprotease-9 (MMP-9), transforming growth factor-beta1 (TGF-beta1), interleukin-6 (IL-6), and macrophage migration inhibitory factor (MIF) were determined using the bromodeoxyuridine proliferation assay and enzyme-linked immunosorbent assay, respectively. RESULTS: Proliferation rate of PMBCs was suppressed by cysteamine in a dose-dependent manner (p = 0.019). Fluorescence of DCF-DA decreased depending on cysteamine concentration (p < 0.001). MMP-9, IL-6 and TGF-beta1 levels were suppressed by cysteamine in a dose-dependent manner (p < 0.05), whereas MIF levels increased with cysteamine concentration of 0.5-10 mM (p = 0.008). CONCLUSIONS: These study results indicate that cysteamine induced the ROS-mediated inhibition of inflammatory cytokine release and proliferation of PBMCs stimulated by chemically injured keratocytes. Thus, cysteamine can be used in the treatment of chemical corneal burns.
