Localization and mRNA Expression of Angiogenin in Bovine Ovary.
- Author:
Heungshik S LEE
1
;
In se LEE
;
Tae Cheon KANG
;
Jehoon SEO
;
Seung Hoon SONG
Author Information
1. Department of Anatomy, College of Veterinary Medicine, Seoul National University, Korea.
- Publication Type:Original Article
- Keywords:
angiogenin;
ovary;
immunohistochemistry;
in situ hybridization;
Korean native cattle;
bovine
- MeSH:
Angiogenesis Inducing Agents;
Animals;
Cattle;
Corpus Luteum;
Endothelial Cells;
Epithelial Cells;
Female;
Fibroblast Growth Factor 1;
Fibroblast Growth Factor 2;
Fibroblasts;
Immunohistochemistry;
In Situ Hybridization;
Menstrual Cycle;
Muscle, Smooth, Vascular;
Oocytes;
Ovarian Follicle;
Ovary*;
RNA, Messenger*;
Theca Cells
- From:Korean Journal of Anatomy
1999;32(1):25-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Ovary is one of the organs in which angiogenesis occurs during ovarian cycle. Angiogenesis is associated with angiogenic factor like acidic fibroblast growth factor, basic fibroblast growth factor and transformation growth factor. Therefore, we performed this study to identify the distribution and mRNA expression of angiogenin, new potential angiogenic factor, in ovary of Korean native cattle by immunohistochemistry and in situ hybridization. Angiogenin immunoreactivity and mRNA expression were observed in endothelial cells, fibroblast and vascular smooth muscle cells. However, we could not observed angiogenin immunoreactivity and mRNA expression in primordial ovarian follicle. In follicular epithelial cells of primary ovarian follicle, weak angiogenin immunoreactivity and mRNA expression were observed. Follicular epithelial cells, theca interna and externa in secondary ovarian follicles, showed angiogenin immunoreactivity, while follicular epithelial cells did the weak mRNA expression. Angiogenin immunoreactivity and mRNA expression were observed in follicular epithlial cells, theca interna and oocyte in tertiary ovarian follicle. The corpus luteum showed strong immunoreactivity and mRNA expression but atretic follicle weak. However, these angiogenin immunoreactivity and mRNA expression became to be weaker during regression. These results suggest that angiogenin may play a role as not only an angiogenic factor but a growth factor in ovary.
