Autoradiographic Study on the Effect of AG60 to the DNA Synthesis of Gastric Epithelium of Mouse Inoculated with Ehrlich Carcinoma.
- Author:
Kyung Ho PARK
1
;
E Tay AHN
;
Jeong Sik KO
;
Sang Sun HA
;
Young Sin CHUNG
;
Eun Kyung HONG
;
Bo Im YOO
;
Sang Geon KIM
;
Kyung Yung LEE
;
Jong Koo KANG
;
Young Bok HAN
Author Information
1. Department of Anatomy, College of Medicine, Soonchunhyang University, Chunan, Korea.
- Publication Type:Original Article
- Keywords:
Acriflavine-guanosine composition;
laveling indicies;
gastric epithelium;
autoradiography
- MeSH:
Animals;
Autoradiography;
Edible Grain;
DNA*;
Epithelial Cells;
Epithelium*;
Formaldehyde;
Gastric Mucosa;
Guanosine;
Injections, Subcutaneous;
Mice*;
Seoul;
Thymidine;
Veins
- From:Korean Journal of Anatomy
1999;32(1):95-104
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To study the tumor-suppression effect of a newly developed anti-tumor agent AG60 [acriflavine (1) : guanosine (1) composition, Taerim Pharm. Co., Seoul, Korea], each Ehrlich carcinoma (10(7) cells)-inoculated mouse received the subcutaneous injection of 0.2 ml of saline, 5 mg/kg of AG60, and 30 mg/kg of AG60 per day for a week. The day following the last injection, each mouse was injected with a single dose of 0.7 microcurie/g of methyl-3H-thymidine (25Ci/mmol, Amersham Lab., England) through tail vein. Seventy minutes after the thymidine injection, animals were sacrificed, and gastric tissues were collected and fixed in 10% neutral formalin. Tissue blocks were washed, dehydrated, embedded and cut in 6 micrometer-thick sections. Deparaffinized sections were coated with autoradiographic emulsion EM 1 (Amersham Lab. England) in a dark room and dried and were placed in a light-tight box. The sections were exposured for 5 weeks in the dark room, and were then developed in D-19 developer. Labeled indices (mean number of labeled cells per 100 epithelial cells in the isthmus) were observed and calculated. The results are as follows; 1. On histological study, gastric mucosa had no morphological changes following the injection of AG60. 2. On autoradiographic study, labeled grains of 3H-thymidine were restricted on the isthmus portion of the gatric gland. 3. On autoradiographic study, labeling indicies of gastric epithelial cells of normal control, experimental control, AG60 (5 mg/kg)-treated and AG60 (30 mg/kg)-treated groups were 21.9+/-0.28%, 18.8+/-0.03%, 21.6+/-1.61% and 6.3+/-0.93%, respectively. These result suggest that AG60 is expected as one of most effective anticancer drugs, and the dosage under 5 mg/kg of AG60 does not result any defect on the DNA synthesis in gastric epithelial cells.
