15-Lipoxygenase-1 Induced by Interleukin-4 Mediates Apoptosis in Oral Cavity Cancer Cells.
- Author:
Jeong Hong KIM
1
;
Jeung Gweon LEE
;
Joo Heon YOON
;
Kun Wayn LEE
;
Hyung Seok SEO
;
Kyung Su KIM
Author Information
1. Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Interleukin-4;
Arachidonate 15-Lipoxygenase;
Apoptosis;
Mouth Neoplasms
- MeSH:
Adenosine Diphosphate Ribose;
Apoptosis*;
Arachidonate 15-Lipoxygenase;
Blotting, Western;
Cell Proliferation;
Cyclooxygenase 2;
Flow Cytometry;
Interleukin-4*;
Mouth Neoplasms;
Mouth*;
RNA, Messenger;
Sensitivity and Specificity
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2005;48(12):1512-1518
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: In oral cavity cancer (OCC) cells, the effects of interleukin-4 (IL-4) are various according to the cell specificity. However, if IL-4 induces apoptosis on OCC cells, the mediator of this apoptosis is uncertain. Therefore, we investigated whether apoptosis of OCC cells occurs by IL-4 and whether 15-lipoxygenase-1 (15-LO-1) induced by IL-4 is the possible mediator of this apoptosis. MATERIALS AND METHODS: SCC 1483 cells were used. Flow cytometry and poly ADP-ribose polymerase cleavage were used to examine apoptosis. Western blot analysis and reverse transcription-polymerase chain reaction were used to measure 15-LO-1 protein and mRNA. RESULTS: The inhibition of cell proliferation by more than 50% was noted from 10 ng/ml of IL-4. At this dose, apoptosis was observed and this apoptosis was inhibited by 2.2 microM caffeic acid. 15-LO-1 expression was observed from the 8 hour treatment of IL-4 and apoptosis increased after the 24 hour treatment of IL-4. In this apoptosis, caspase cascade, cyclooxygenase-2, and non-steroidal anti-inflammatory drugs-activated gene-1 (NAG-1) were not involved. CONCLUSION: IL-4 induced apoptosis in SCC 1483 OCC cells and 15-LO-1 induced by IL-4 may mediate this apoptotic pathway.
