Apoptotic Effect of Co-treatment with Chios Gum Mastic and HS-1200 on G361 Human Melanoma Cell Line.
- Author:
Young Joo HUR
1
;
Young Ki KIM
;
Hyun Ho KWAK
;
Gyoo Cheon KIM
;
Seung Eun LEE
;
In Ryoung KIM
;
Chul Hoon KIM
;
Bong Soo PARK
Author Information
1. Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Korea. parkbs@pusan.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Chios gum mastic (CGM);
Synthetic bile acid;
HS-1200;
Apoptosis;
Human melanoma cells (G361 cells)
- MeSH:
Apoptosis;
Bile Acids and Salts;
Caspase 3;
Caspase 9;
Cell Line;
Chenodeoxycholic Acid;
Cytochromes c;
Cytosol;
DNA;
DNA Fragmentation;
Electrophoresis;
Exudates and Transudates;
Flow Cytometry;
Gingiva;
Humans;
Melanoma;
Microscopy, Confocal;
Pistacia;
Proteasome Endopeptidase Complex;
Resins, Plant;
Trees;
Up-Regulation
- From:Korean Journal of Anatomy
2009;42(2):83-92
- CountryRepublic of Korea
- Language:English
-
Abstract:
Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induce apoptosis in a few cancer cells in vitro. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of cotreatment with a natural product, CGM and a CDCA derivative, HS-1200 on G361 human melanoma cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of G361 cells, MTT assay was conducted. To investigate augmentation of apoptosis in G631 cells co-treated with CGM and HS-1200, DNA electrophoresis, Hoechst staining, proteasome activity assay, flow cytometry, Westen blot analyses, immunofluorescent staining and confocal microscopy were performed. In this study, G361 cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA content, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, activation of caspase-9, caspase-3, PARP and DFF45 (ICAD), and up-regulation of Bax whereas each single treated G361 cells did not. Although the single treatment of 40 micro/mL CGM or 25 micro HS-1200 for 24 hrs did not induce apoptosis, the co-treatment of them induced prominently apoptosis. Therefore, combination therapy of CGM and HS-1200 could be considered, in the future, as an alternative therapeutic strategy for human melanoma.
