Expression of the p75NTR Neurotrophin Receptor in the Thymic Medullary Epithelial Cells and Interdigitating Dendritic Cells during Rat Thymus Regeneration Following Acute Thymic Involution.
- Author:
Na Ri SHIM
1
;
Hyo Jin KIM
;
Sae Ock OH
;
Sun Yong BAEK
;
Bong Seon KIM
;
Jae Bong KIM
;
Sik YOON
Author Information
1. Department of Anatomy, Pusan National University School of Medicine, Yangsan, Korea. sikyoon@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
p75NTR;
Thymic medullary epithelial cells;
Interdigitating dendritic cells;
Thymus regeneration;
Neuroimmune interaction
- MeSH:
Aluminum Hydroxide;
Animals;
Blotting, Western;
Carbonates;
Cyclophosphamide;
Dendritic Cells;
Epithelial Cells;
Fluorescent Antibody Technique;
Immune System;
Rats;
Regeneration;
RNA, Messenger;
T-Lymphocytes;
Thymocytes;
Thymus Gland
- From:Korean Journal of Anatomy
2009;42(2):105-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
The existence of a functional link between the nervous and immune systems has been well established. The present study was to characterize the expression of p75NTR during thymus regeneration from acute involution induced by cyclophosphamide in the rat. Immunohistochemical and double immunofluorescence analyses demonstrated that expression of the p75NTR was decreased in the thymic medullary epithelial cells and interdigitating dendritic cells during thymus regeneration. The presence of p75NTR protein in extracts from the control and regenerating rat thymus was confirmed by western blot. Furthermore, RT-PCR analysis supported these results by demonstrating that thymic extracts contain p75NTR mRNA at lower levels during thymus regeneration. Thus, our results suggest that the p75NTR located on the thymic medullary epithelial cells and interdigitating dendritic cells could play a role in the development of new T cells to replace the thymocytes damaged during thymus regeneration
