IL-1beta Mediated COX-2 Expression in Human Airway Epithelial Cells.
- Author:
Yong Dae KIM
1
;
Si Youn SONG
;
Eun Jin KWON
;
Suk Hwan BAEK
;
Gil Sung CHO
;
Hak Soo KIM
;
Seok Keun YOON
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Taegu, Korea. ydkim@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Interleukin-1beta;
Cyclooxygenase-2;
Prostaglandin E2;
NCI-H292 cells
- MeSH:
Blotting, Western;
Cycloheximide;
Cyclooxygenase 2;
Dactinomycin;
Dinoprostone;
Epithelial Cells*;
Gene Expression;
Humans*;
Interleukin-1beta;
Prostaglandins;
Prostaglandins I
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2002;45(2):132-136
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Prostaglandin is one of the important inflammatory mediator in inflammatory diseases. Cyclooxygenase-2 (COX-2) plays a key role in biosynthesis of prostaglandins. In this study, we aimed to investigate COX-2 expression and prostaglandin E2 (PGE2) production by interleukin-1beta (IL-1beta) in cultured human airway epithelial cells. MATERIALS AND METHOD: COX-2 gene expression, and COX-2 protein, PGE2 production by IL-1beta were analyzed by RT-PCR, Western blot, and enzymeimmunoassay (EIA) in cultured human airway NCI-H292 epithelial cells. RESULTS: The COX-2 protein production was increased when the cells were exposed to IL-1beta in a dose dependent manner. The maximum level of COX-2 protein was detected at 20 ng/ml of IL-1beta. After 4 hours, the production of COX-2 protein was detected by IL-1beta(20 ng/ml) and this was held up to 12 hour. The maximum level of COX-2 protein production reached at 8 hour of exposure to IL-1beta and this was held up to 12 hour. The release of PGE2 occurred in the same pattern as the IL-1beta-mediated COX-2 protein production. The COX-2 gene expression was induced by IL-1beta (20 ng/ml). The IL-1beta-mediated COX-2 expression was suppressed by actinomycin D, but was not affected by cycloheximide. CONCLUSION: These results suggest that the IL-1beta-mediated COX-2 expression and the PGE2 production were increased in dose and time dependent manner and regulated in the transcriptional step.
