Effect of Inhibitor of TNF-alpha and Oxatomide on Experimental Immune Mediated Otitis Media with Effusion.
- Author:
Hyeon Jin AUO
1
;
Yong Soo PARK
;
Eun ju JEON
;
Sang Won YEO
;
Ki Hong CHANG
;
Seung Kyun LEE
Author Information
1. Department of Otolarngology-HNS, College of Medicine, The Catholic University of Korea, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Otitis media with effusion;
Tumor necrosis factor receptor;
Hemocyanin;
Leukotriene antagonists
- MeSH:
Animals;
Capillary Permeability;
Ear;
Ear, Middle;
Evans Blue;
Hemocyanin;
Immunization;
Inflammation;
Leukotriene Antagonists;
Mucous Membrane;
Otitis Media with Effusion*;
Otitis Media*;
Otitis*;
Rats;
Receptors, Tumor Necrosis Factor;
Sodium Chloride;
Temporal Bone;
Tumor Necrosis Factor-alpha*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2004;47(6):507-514
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Local immune response, one of the pathogenesis of middle ear effusion (MEE) is associated with the development and persistence of effusion in the middle ear cavity and inflammatory mediators play a major role in the production of MEE. The purpose of this study was to determine the effects of TNF-alpha antagonist and oxatomide on the outcome of immune mediated otitis media with effusion (OME) in rats. MATERIALS AND METHOD: Otitis media was induced by injecting KLH (Keyhole lympet hemocyanin) transtympanically 7 days after systemic immunization. Phosphate-buffered saline solution was used as control. Other groups were pretreated with TNF-alpha antagonist (soluble TNF receptor type I, sTNF RI) or oxatomide respectively before transtympanic injection of KLH. Seventy-two hours after the transtympanic injection, temporal bones in each group were examined histopathologically and vascular permeability of the middle ear mucosa was measured by the Evans blue vital dye technique. RESULTS: In the KLH, sTNF RI and oxatomide groups, MEE was developed in 83%, 0%, 66% of the ears, respectively. The oxatomide group and sTNF RI group showed significant decrease in inflammation, mucosal thickening and vascular permeability as compared with KLH group and those parameters of sTNF RI group showed lower values than those of oxatomide group. CONCLUSION: Transtympanic administration of sTNF RI and oxatomide appears to suppress the development of immune mediated OME. In terms of inhibiting MEE, sTNF RI was more effective than oxatomide. This study suggests that TNF-alpha antagonist and oxatomide may have a adjunctive role in the treatment and prevention of otitis media with effusion.
