Cyclooxygenase-2 Expression in Bile Duct Cancer.
- Author:
Kyu Hee HER
1
;
Sun Whe KIM
;
Jin Young JANG
;
Yoo Seok YOON
;
Kwang Sik KIM
;
Hye Seung LEE
;
Yong Bum YOON
;
Yong Hyun PARK
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. sunkim@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Bile Duct Neoplasms;
Cyclooxygenase;
Immunohistochemistry;
Western Blotting
- MeSH:
Bile Duct Neoplasms*;
Bile Ducts*;
Bile*;
Blotting, Western;
Carcinogenesis;
Colonic Neoplasms;
Colorectal Neoplasms;
Cyclooxygenase 2*;
Cytoplasm;
Eating;
Epithelial Cells;
Humans;
Immunohistochemistry;
Inflammation;
Polymerase Chain Reaction;
Prostaglandin-Endoperoxide Synthases;
Reverse Transcription;
RNA, Messenger
- From:Korean Journal of Hepato-Biliary-Pancreatic Surgery
2004;8(3):172-179
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Epidemiological studies have shown that regular ingestion of a nonsteroidal anti-inflammatory drug reduces the risk of fatality from colon cancer. Cyclooxygenase-2 (COX-2) has been found to be overexpressed in various types of tumor, including colorectal cancer. It has been suggested that the COX-2 enzyme may play an important role in carcinogenesis and tumor progression. The aims of this study were to find the relationship between COX-2 expression and bile duct carcinogenesis and its clinical significance in bile duct cancer. METHODS: The COX-2 expression was determined using three methods, reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemical staining. mRNA and proteins were extracted from the tissue specimens of 22 bile duct cancer patients. RESULTS: RT-PCR and western blotting found COX-2 expressions in all the cancers and their paired noncancerous tissues. Immunohistochemistry revealed the highest levels of COX-2 expressed in bile duct carcinoma cells, mainly in the cytoplasm, and a weak reactivity of the COX-2 protein was observed in noncancerous bile duct epithelial cells. CONCLUSION: Based on this study, it is postulated that the carcinogenesis mechanism of COX-2 expression may be initiated from a noncancerous condition, such as chronic inflammation, and may advance to bile duct cancer progression.
