Effects of Anti-Inflammatory Drugs on the Interleukin-1 beta-Induced Cyclooxygenase-2 Expression in Human Airway Epithelial Cells.
- Author:
Yong Dae KIM
1
;
Hyun Jae WOO
;
Chang Hoon BAI
;
Jae Heun SIN
;
Young Jung SEO
;
Kang Shik PARK
;
Si Yeon SONG
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Cyclooxygenase;
Interleukin-1;
Inhibitor
- MeSH:
Adrenal Cortex Hormones;
Blotting, Western;
Budesonide;
Cyclooxygenase 2*;
Dexamethasone;
Epithelial Cells*;
Humans*;
Indomethacin;
Interleukin-1*;
Interleukin-1beta;
Prostaglandin-Endoperoxide Synthases;
Prostaglandins;
RNA, Messenger;
Triamcinolone
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2004;47(10):988-993
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Cyclooxygenase-2 (COX-2) is a key molecule in the biosynthesis of prostaglandins, which are important inflammatory mediators in human airway inflammatory diseases. This study was designed to investigate the effects of several COX inhibitors on the interleukin-1beta (IL-1beta)-mediated COX-2 expression in human airway epithelial cells. MATERIALS AND METHOD: We observed the effects of anti-inflammatory drugs such as budesonide, triamcinolone, dexamethasone, NS-398, indomethacin, salicylate and resveratrol on the IL-1beta-induced COX-2 expression in cultured human airway NCI-H292 epithelial cells. The levels of COX-2 mRNA and COX-2 protein were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. RESULTS: NS398, reveratrol and three corticosteroids strongly suppressed the IL-1beta-mediated COX-2 expression. However, indomethacin and salicylate did not inhibit or inhibited only weakly. CONCLUSION: The extent of IL-1beta-induced supression of COX-2 expression in the cultured human airway NCI-H292 epithelial cells depended on the kinds of anti-inflammatory drugs.
