Effects of cis - Platin on the Type I Collagen of the Osteoid in the Metaphysis of Rat Tibia.
- Author:
Joo Hee HAN
;
Jong Heon KIM
;
Tae Seung KIM
;
Jae Lim CHO
;
Ho Sam CHUNG
- Publication Type:Original Article
- Keywords:
Rat tibia;
Metaphysis;
Osteoid;
Type I collagen;
cis-Platin
- MeSH:
Animals;
Collagen;
Collagen Type I*;
Goats;
Immunoglobulin G;
Rats*;
Tibia*
- From:The Journal of the Korean Orthopaedic Association
1997;32(4):911-918
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The present study has been undertaken to pursue the cytotoxic effects of cis-Platin on the osteoid formation in metaphysis of rat tibia. By using the immunohistological staining method for type I collagen in rat tibial osteoid, the author detected the deposition of type I collagen, which is the collagenous constituent of endochondral osteoid, after administration of cis-Platin in experimental animals. For the immunological reactions of type I collagen, we used the rabbit anti-rat collagen type I polyclonal antibody as primary antibody and biotinylated goat anti-rabbit IgG as secondary antibody. The distributions of immunohistological reactions in the each of metaphyseal osteoids were analyzed with an image analyzer, and we studied the variances of type I collagens by statistical probabilities. In 12 hours after cis-Platin injection, immunoreactive area in the osteoid of metaphysis was distinctly decreased. Immunoreactive area of type I collagen in osteoids of 1 day and 3 days group metaphysis was increased more than that of 12 hours group and the type I collagen in the metaphysis showed weak immunoreactions of type I collagens with an image analyzer. In the osteoids of 7 days group after cis-Platin injection, the immunoreactive area was similar to that of control group. It is consequently suggested that cis-Platin would induce the decrease of type I collagen in the osteoid. But the type I collagen in tibial osteoid shows the increase from a few days after cis-Platin injection.
