Expression of HSP27 and alphaB-crystallin in Avian Cerebellum during Development.
- Author:
Je Hoon SEO
1
;
Sang Won MOON
;
Hyun Joon SOHN
Author Information
1. Department of Anatomy College of Medicine, Chungbuk National University, Cheongju, Korea. seojh@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Small heat shock protein;
HSP27;
alphaB-crystallin;
Developing cerebellum;
Purkinje cell;
Oligodendrocyte;
Cerebellar nucleus
- MeSH:
Apoptosis;
Cerebellar Nuclei;
Cerebellum*;
Embryonic Structures;
Fluorescent Antibody Technique;
Hand;
Heat-Shock Proteins, Small;
Molecular Chaperones;
Myelin Sheath;
Neuroglia;
Neurons;
Oligodendroglia;
Purkinje Cells;
Stress, Physiological
- From:Korean Journal of Anatomy
2006;39(6):453-460
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It is well known that small heat shock proteins play a role as molecular chaperone. However, during normal development of the cerebellum, expression and distribution of HSP27 and alphaB-crystallin (alphaBC) which are small heat shock proteins have not been reported. To verify the protective role of HSP27 and alphaBC in neurons and glial cells, we examined the expression and distribution of HSP27 and alphaBC in the developing chick cerebellum using immunoblot, immunohistochemical and double immunofluorescence staining. Expression of both HSP27 and alphaBC was first identified in the cerebellum of the embryonic day 14 (E14) embryo, and was increased at E18. Double immunofluorescence analysis with myelin-basic protein (MBP) demonstrated that alphaBC positive (+) cells were mature myelinating oligodendrocytes. alphaBC+ cells were observed in the white matter of the E14 cerebellum. At E18, there were a number of alphaBC+ cells in the white matter and a few cells in the granular layer of the gray matter. On the other hand, HSP27+ cells were observed in the white matter and the Purkinje cell layer at E14. At E18, HSP27+ signals were observed in Purkinje cells and neurons of cerebellar nucleus as well as oligodendrocytes in the white matter and the granular layer. The results that HSP27 and alphaBC were expressed in specific neurons and glial cells in the developing cerebellum suggest that HSP27 and alphaBC may be involved in the protective mechanism for the apoptosis of neurons and the physiological stress occurred in oligodendrocyts during cell maturation.
