Role of Toll-like Receptor 2 in a Mouse Model of Allergic Rhinitis.
- Author:
Jun Myung KANG
1
;
Jin Hee CHO
;
Chang Eun SONG
;
Chan Soon PARK
;
Byung Guk KIM
;
Sung Won KIM
;
Joo Hyung LEE
;
Seung Ho CHOI
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea. entkjm@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Toll-like receptor 2;
Allergic rhinitis;
Animal model
- MeSH:
Animals;
Aspergillus fumigatus;
Bias (Epidemiology);
Eosinophils;
Immunity, Innate;
Immunoglobulin E;
Injections, Intraperitoneal;
Interferons;
Interleukin-10;
Interleukin-4;
Interleukin-5;
Interleukins;
Mice*;
Models, Animal;
Nasal Lavage Fluid;
Rhinitis*;
Spleen;
Toll-Like Receptor 2*;
Toll-Like Receptors*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2007;50(11):989-995
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Mammalian Toll-like receptors (TLR) participate in innate immune responses to microbial pathogens. Recent interest has been focused on the concept that TLR-induced innate responses can modulate subsequent adaptive immune responses. The objective of this study is to determine whether TLR 2 stimulation in vivo would modulate subsequent allergic responses in an Aspergillus fumigatus (Af) murine model of allergic rhinitis (AR). MATERIALS AND METHOD: Mice were sensitized via intraperitoneal injection with Af antigen and alum, and received a series of three daily intranasal Af antigen challenge. A TLR 2 agonist, PamCys was administrated intranasally one day before sensitization or one day before the first intranasal allergen challenge. Adaptive immune profiles and response to Af challenge were assessed. RESULTS: PamCys decreased the allergen induced nasal recruitment of eosinophils and interleukin (IL)-5 in nasal lavage fluids compared with mice treated with no PamCys. Serologic data revealed that PamCys downregulated Af-specific IgE in the sera of PamCys-treated mice. In addition, spleen cells from the PamCys-treated mice displayed attenuated Af-specific IL-4 and IL-5, but increased interferon (IFN)- and immunosuppressive IL-10. CONCLUSION: The present study demonstrate that TLR 2 agonist decreases allergic responses in this AR model by shifting T helper 2 (Th2) biased immune parameters towards Th1 dominance.
