Effect of Platelet-Activating Factor and PAF-Antagonist on the Mucociliary Clearance of the Eustachian Tube in Guinea Pigs.
- Author:
Chung Ku RHEE
1
Author Information
1. Department of Otolaryngology, Dankook University College of Medicine, Cheonan, Korea. DKUHENT@unitel.co.kr
- Publication Type:Original Article
- Keywords:
PAF;
PAF-antagonist;
Mucociliary clearance function;
Eustachian tube
- MeSH:
Animals;
Cilia;
Eustachian Tube*;
Guinea Pigs*;
Guinea*;
Mucociliary Clearance*;
Mucus;
Otitis Media;
Platelet Activating Factor
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1999;42(3):273-278
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The purpose of this study was to test whether platelet activating factor (PAF) impairs the mucociliary clearance function of the eustachian tube (ET)in dose dependent manner and PAF-antagonist can prevent the impairment of mucociliary function of the ET induced by PAF. RESULTS: Coomassie Brilliant Blue (CBB) dye transport time (DTT) in normal guinea pigs was 69 seconds. The dye transport time after the applications of normal saline, PAF 1, and 2 ug/ml of PAF into the bullae were 66, 74, and 157 seconds, respectively. The time was over 15 minutes when 4, 8, and 16 ug/ml of PAF were applied. The DTT were 62 seconds when the animals were pretreated with PAF-antagonist (WEB 2170). There were significant delay in the DTTs after treatment with 2, 4, 8, and 16 ug/ml of PAF. Histopathological examination of ETs from groups with significant delay in DTTs showed intact cilia, mucus plugs, increased inflammatory cells, and exfoliation of cells. CONCLUSION: This study demonstrated that PAF impaired mucociliary clearance function of the eustachian tube in dosedependent manner. This impairment of mucociliary clearance function was prevented by pre-treatment with PAF-antagonist. The findings of this study suggest that PAF plays an important role in the pathogenesis of otitis media by impairing eustachian tube clearance function.
