Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea.
- Author:
Hyun Ho CHO
1
;
Sang Hyun KIM
;
Wongi MIN
;
Bok Kyung KU
;
Yong Hwan KIM
Author Information
1. Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea.
- Publication Type:Original Article
- Keywords:
adherence/invasion ability;
Campylobacter spp.;
dog;
Guillain-Barre syndrome-associated genes;
virulence genes
- MeSH:
Animals;
Breeding;
Busan*;
Campylobacter*;
Dogs*;
Family Characteristics;
Humans*;
Korea*;
Polymerase Chain Reaction;
Prevalence*;
Virulence*
- From:Korean Journal of Veterinary Research
2014;54(1):39-48
- CountryRepublic of Korea
- Language:English
-
Abstract:
The prevalence of thermophilic Campylobacter (C.) spp. in stray, breeding, and household dogs was 25.2, 12.0, and 8.8%, respectively. C. jejuni and C. upsaliensis were the predominant Campylobacter spp. from household dogs. cdtA, cdtB, and cdtC were detected by PCR in all isolates. Despite the high cytolethal distending toxin (CDT) gene prevalence, only 26 (31%) C. jejuni strains and one (15.3%) C. coli strain showed evidence of CDT production in HEp-2 cell cytotoxicity assays. Virulence-associated genes detected in the C. jejuni and C. coli isolates were cadF, dnaJ, flaA, racR, ciaB, iamA, pldA, virB11, ceuE, and docC. cadF, dnaJ, flaA, and ceuE were found in all C. jejuni and C. coli isolates. When detecting Guillain-Barre syndrome-associated genes (galE, cgtB, and wlaN), galE was identified in all isolates. However, cgtB and wlaN were more prevalent in C. jejuni isolates from humans than those from dogs. Adherence and invasion abilities of the C. jejuni and C. coli strains were tested in INT-407 cells. A considerable correlation (adjusted R2 = 0.678) existed between adherence and invasion activities of the Campylobacter spp. isolates.
