Effects of Portulaca Oleracea L. Extract on Lipolysis and Hormone Sensitive Lipase (HSL) Gene Expression in 3T3-L1 Adipocytes.
- Author:
Mak Soon LEE
1
;
Chong Tai KIM
;
Chul Jin KIM
;
Young Jin CHO
;
Yangha KIM
Author Information
1. Department of Food and Nutritional Sciences, Ewha Womans University, Seoul 120-750, Korea. yhmoon@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Portulaca oleracea L;
3T3-L1 adipocytes;
lipolytic effect;
HSL gene expression
- MeSH:
Adipocytes*;
Fatty Acids, Nonesterified;
Gene Expression*;
Glycerol;
Lipolysis*;
Portulaca*;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Sterol Esterase*;
Up-Regulation
- From:The Korean Journal of Nutrition
2006;39(8):742-747
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to investigate the lipolytic effects of Portulaca oleracea L. extract in 3T3-L1 adipocytes. The Portulaca oleracea L. was extracted with extrusion method using twin-screw extruder under 58~60 rpm screw speed, 4~5 kg/hr feed rate, 140degrees C extrusion temperature. The lipolytic action of Portulaca oleracea L. extract was estimated by measuring the amount of glycerol and free fatty acids (FFA) released from 3T3-L1 adipocytes and by measuring the cellular lipid content in 3T3-L1 adipocytes. The hormone sensitive lipase (HSL) mRNA level was analyzed using quantitative real-time PCR. The Portulaca oleracea L. extract at 1 to 100 microgram/ml suppressed lipid accumulation. The release of glycerol and FFA into the medium, and the mRNA level of HSL were significantly increased by the addition of Portulaca oleracea L. extract at dose-dependent manner. In conclusion, the Portulaca oleracea L. extract was suggested to have the lipolytic effect through release of lipolytic products (FFA and glycerol) of triacylglyceride to the culture medium and suppression of lipid accumulation via up-regulation of HSL gene expression in 3T3-L1 adipocytes.
