Formaldehyde Gas Induced Morphological Changes in the Rat Olfactory Epithelium.
- Author:
Sun Yong BAEK
1
;
Jae Ki JEON
;
Yong Suk MOON
;
Seung Young HWANG
;
Hwan Jung ROH
;
Eui Kyung GOH
;
Soo Geun WANG
;
Kyong Myong CHON
;
Bong Seon KIM
;
Jae Bong KIM
;
Jin Jeong KIM
Author Information
1. Department of Anatomy and 1Otolaryngology, College of Medicine, Pusan National University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
olfactory epithelium;
formaldehyde gas;
lectin histochemistry;
olfactory stem cells;
BrdU
- MeSH:
Animals;
Atrophy;
Bromodeoxyuridine;
Epithelial Cells;
Formaldehyde*;
Glycoproteins;
Immunohistochemistry;
Inhalation;
Lectins;
Metaplasia;
Olfactory Mucosa*;
Rats*;
Stem Cells
- From:Korean Journal of Anatomy
1998;31(3):327-340
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Experimental animals were inhalated 50 ppm formaldehyde gas for 3 times with one hour exposure and one hour rest. The olfactory mucosa were taken from the animals on 4, 7, 9, 11 days and 2-6 weeks after the inhalation. The characteristics of the various glycoproteins and the mitotic activity of the olfactory epithelial cells were investigated using lectins and 5'-bromodeoxyuridine (BrdU, 50 mg/kg) which injected one hour before sacrifice of the animals. The results were as follows; 1. In experimental animals, the degenerative changes of the olfactory epithelium such as atrophy and squamous metaplasia were observed until 2 weeks after formaldehyde gas inhalation. 2. In control animals, positive reactions appeared in the supporting cell to PNA, SBA, WGA, ECL, PHA-L and in the olfactory cells to PNA, SBA, WGA, UEA and in the proper basal cell to GS-I, SBA, WGA, PHA-L. In experimental groups, the positive reaction was increased in the supporting cells to SBA, ECL, PHA-L and in Bowman's gland to used lectins except ECL, GS-I. 3. The number of BrdU labelled cells in the olfactory epithelium was 14.8+/-1.2/mm in the control animals. The mitotic activities were decreased to 4.8+/-0.8 mm in 2 weeks and recovered in 3 weeks after the gas inhalation. 4. To find the stem cells of olfactory receptor cells, double labelling method was performed with lectins which were specific for proper basal cells (GS-I or PHA-L) and BrdU immunohistochemistry. The ratio of globose/proper basal cells in BrdU labelled cells with GS-I lectin positive reaction was 82%/18% in control group, 39.3%/60.7% in lesion group and 55.5%/44.5% in recovery group.
