The Effect of MPTP on Movement and TH-immunoreactive Neuronal Changes of C57BL/6 Mice in Relation to Aging.
- Author:
Jae Woo KIM
1
;
Sun Yong BAEK
;
Hae Won YOON
Author Information
1. Department of Neurology, Dong-A University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Parkinson's disease;
MPTP;
C57BL/6 mice;
tyrosine hydroxlase
- MeSH:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine*;
Aging*;
Animals;
Immunohistochemistry;
Locus Coeruleus;
Mice*;
Models, Animal;
Motor Activity;
Neurons*;
Parkinson Disease;
Substantia Nigra;
Tyrosine 3-Monooxygenase;
Ventral Tegmental Area
- From:Korean Journal of Anatomy
1999;32(3):421-424
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effects of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) on movement and tyrosine hydroxylase (TH)-immunoreactive (ir) neuronal changes in young (5~6 weeks) and aged (10~12 months) C57BL/6 mice were studied. Locomotor activity was measured during 180 minutes after a single injection of 30 mg/kg of MPTP. For immunohistochemistry both young and aged mice were injected four repeated dosages of 10 mg/kg of MPTP 12 hours apart. We counted the numbers of TH-ir cell bodies using immunohistochemical technique in substantia nigra (SN), ventral tegmental area (VTA) and locus ceruleus (LC) 7 days after the last injection of MPTP. There was a marked decrease of locomotor activity in MPTP-treated young and aged mice, and a delay in recovery of locomotor activity in MPTP-treated aged mice. In young mice, there was a decrease in the number of TH-ir cell bodies in the SN of young mice, but not in VTA or LC. In aged mice, there was a significant decrease in the number of TH-ir cell bodies in VTA as well as SN. It was concluded that aged mice were more sensitive to MPTP than young mice, and MPTP-treated aged mice a more useful animal model for studing the characteristics of Parkinson's disease.
