The Effect of Caffeine on 3T3-L1 Adipocyte Differentiation : A Nutrigenomical Approach.
- Author:
Mi Ja KIM
1
;
Youngok KIM
;
Joo Ho CHUNG
;
Jong Woo KIM
;
Hye Kyung KIM
Author Information
1. Department of Obesity Management, Graduate School of Obesity Science Dongduk Women's University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
nutrigenomics;
caffeine;
3T3-L1;
obesity
- MeSH:
Adipocytes*;
Caffeine*;
Coffee;
Collagen;
Colony-Stimulating Factors;
Complement Factor D;
Cytokines;
Gene Expression;
Genome, Human;
Granulocyte-Macrophage Colony-Stimulating Factor;
Granulocytes;
Humans;
Interleukin-12;
Interleukin-13;
Interleukin-3;
Nutrigenomics;
Obesity;
Oxidoreductases;
Protein Array Analysis;
Tea;
Vascular Endothelial Growth Factor A
- From:The Korean Journal of Nutrition
2005;38(8):649-655
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Nutrigenomics refers to research that investigates the interaction between nutrition and the human genome. Caffeine in tea and coffee is widely and routinely consumed by people. This study was performed to confirm the effect of caffeine treatment on the gene expression and cytokine profiling in 3T3-L1 adipocyte cells using microarray and protein array methodology. Treatment of caffeine in 3T3-L1 adipocyte cells increased expression of several genes related with obesity including adipocyte C1Q and collagen domain containing (ACDC), Adipsin (ADN), uncoupling protein 3 (UCP3), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is known as lipid storage enzyme, was decreased by caffeine treatment. Furthermore, cytokines, such as interleukin-3 (IL-3), interleukin-12 (IL-12), interleukin-13 (IL-13), granulocyte colony stimulating factor (GCSF), granulocyte macrophage colony stimulating factor (GM-CSF) and vascular endothelial growth factor (VEGF), were decreased in caffeine treated 3T3-L1 adipocyte cells. These results provided interesting information about the genes related with caffeine and cytokine expression profiling in obesity.
