An Immunohistochemical Tracing on the Central Neural Pathways of the Spinal Accessory Nerve using Pseudorabies Virus.
- Author:
Jung Je PARK
1
;
Eui Gee HWANG
;
Tae Gee JUNG
;
Jin Pyong KIM
;
Sea Yuong JEON
Author Information
1. Department of Otolaryngology, College of Medicine, Gyeongsang National University, Chinju, Korea.
- Publication Type:Original Article
- Keywords:
Pseudorabies virus;
Spinal accessory nerve;
Immunohistochemistry
- MeSH:
Accessory Nerve*;
Animals;
Antibodies;
Cerebral Cortex;
Herpesvirus 1, Suid*;
Immunohistochemistry;
Neural Pathways*;
Neurons;
Paraventricular Hypothalamic Nucleus;
Pseudorabies*;
Rats;
Spinal Cord
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2001;44(8):853-858
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVE: The transsynaptic transfer of neurotropic viruses is an effective tool for tracing chains of connected neurons because the replication of virus in the recipient neurons after transfer amplifies the "tracer signal". The purpose of study was to identify the location of spinal nucleus of the accessory nerve and the central neural pathways using Bartha strain of Pseudorabies virus (PRV-Ba), as a transsynaptic tracer. MATERIALS AND METHODS: PRV-Ba was injected into the sternocleidomastoid muscle of a rat, and the localization of PRV-Ba in the rat spinal cord and CNS was identified with light microscopic immunohistochemistry using primary antibodies against the PRV-Ba. RESULTS: Sequential tracing of retrogradely labeled cells was done. The shapes of positive immunoreactive cells were mostly ovoid or polygonal, and were shown in the spinal nucleus of the accessory nerve, nucleus ambiguus, paraventricular nucleus, and the primary motor area of cerebral cortex. CONCLUSIONS: These results showed the location of spinal accessory nucleus and the central neural pathways of spinal accessory nerve using PRV-Ba.
