Effect of Vitamin C, Silicon and Iron on Collagen Synthesis and Break-Down Enzyme Expression in the Human Dermal Fibroblast Cell (HS27).
10.4163/kjn.2009.42.6.505
- Author:
Jeong Eun KIM
1
;
Jinah LEE
;
Hyunae KIM
;
Jungmin KIM
;
Yunhi CHO
Author Information
1. Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 449-701, Korea. choyunhi@khu.ac.kr
- Publication Type:Original Article
- Keywords:
vitamin C;
silicon;
iron;
prolyl hydroxylase;
lysyl hydroxylase
- MeSH:
Ascorbic Acid;
Collagen;
Dermis;
Fibroblasts;
Humans;
Hydrogen-Ion Concentration;
Iron;
Lysine;
Matrix Metalloproteinase 1;
Oxygen;
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase;
Procollagen-Proline Dioxygenase;
Proline;
RNA, Messenger;
Silicon;
Tissue Inhibitor of Metalloproteinase-1;
Vitamins
- From:The Korean Journal of Nutrition
2009;42(6):505-515
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Collagen is the major matrix protein in dermis and consists of proline and lysine, which are hydroxylated by prolyl hydroxylase (PH) and lysyl hydroxylase (LH) with cofactors such as vitamin C, oxygen, iron (Fe2+), ketoglutarate and silicon. The collagen degradation is regulated by matrix metalloproteinase-1 (MMP-1), of which is the major collagen-degrading proteinase whereas tissue inhibitors of metalloproteinase-1 (TIMP-1) bind to MMP-1 thereby inhibiting MMP-1 activity. In this study, we investigated the effects of vitamin C, silicon and iron on mRNA, protein expressions of PH, LH, MMP-1 and TIMP-1. The physiological concentrations of vitamin C (0-100 micrometer), silicon (0-50 micrometer) and iron (Fe2+:0-50 micrometer) were treated to human dermal fibroblast cells (HS27 cells) for 3 or 5days. The expression level of mRNA and protein was increased in not only PH but also LH when cells were incubated with vitamin C. A similar increase in LH mRNA or protein expression occurred when cells were incubated with silicon. Our results suggest that treatment of vitamin C and silicon increased mRNA and protein expression of PH and LH in human dermal fibroblast.
