Increased Expression of the TGF-beta Isoform and Changed Contents of Collagen in Tendon of Cerebral Palsy Patients.
10.4055/jkoa.2004.39.5.531
- Author:
Sung Taek JUNG
1
;
Hyoung Yeon SEO
;
Jae Joon LEE
;
Myung Sun KIM
;
Yang Kyung KIM
;
Gye Jin KIM
Author Information
1. Department of Orthopaedic Sugery, Chonnam National University Medical School, Gwangju, Korea. stjung@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Cerebral palsy;
Achilles tendon;
Transforming growth factor-beta(TGF-beta);
Collagen
- MeSH:
Achilles Tendon;
Cerebral Palsy*;
Clubfoot;
Collagen Type III;
Collagen*;
Contracture;
Humans;
Intercellular Signaling Peptides and Proteins;
Muscle Spasticity;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger;
Tendons*;
Transforming Growth Factor beta*
- From:The Journal of the Korean Orthopaedic Association
2004;39(5):531-536
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study measured the expression level of the transforming growth factor-beta (TGF-beta ) isoform expression and the collagen composition within the Achilles tendon from cerebral palsy (CP) patients. MATERIALS AND METHODS: The Achilles tendons were obtained from 15 CP patients with spastic equinovarus. The presence of the TGF-beta isoform and the composition of the collagen were examined histologically, performing by immunohistochemical staining (IHS) and determining the mRNA expression level using a reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: IHS revealed the presence of TGF-beta 1 and TGF-beta 2 expression in 2/15 cases and 4/15 cases respectively, and weak TGF-beta 3 expression in 7/15 cases. The TGF-beta 1 and TGF-beta 2 expression levels were uniform in all 15 cases according to RT-PCR, while TGF-beta 3 expression was observed in 8 out of 15 cases. IHS and RT-PCR showed strong TGF-beta 3 expression in 6/7 non-ambulatory patients. An immature form of collagen, type III collagen, was observed more abundantly in the non-ambulatory patients. CONCLUSION: These results suggest that contracture in CP may induce changes in the type of collagen via growth factors such as TGF-beta .
