Expression of 15-Lipoxygenase and Cyclooxygenase-2 in Human Nasal Mucosa and Cultured Human Nasal Epithelial Cells.
- Author:
Kyung Su KIM
1
;
Joo Heon YOON
;
Chang Hoon KIM
;
Sun Goo KIM
;
Jung Hyun CHANG
;
Ji Hoon KIM
Author Information
1. Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Nasal mucosa;
15-Lipoxygenase, arachidonate;
Synthase, prostaglandin;
Cell differentiation
- MeSH:
Humans
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2003;46(8):649-653
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The purpose of our study was to examine the expression of 15-lipoxygenase (15-LO) in human nasal mucosa and to investigate the change of expression of 15-LO and cyclooxygenase-2 (COX-2) as a function of mucociliary and squamous differentiation. MATERIALS AND METHOD: Human inferior turbinates were used and immunohistochemistry with 15-LO and COX-2 antibody was done. Passage-2 normal human nasal epithelial cell culture using air-liquid interface method was performed for 14 days and the cells were divided as retinoic acid (RA)-sufficient and RA-deficient group. Western blot analysis for 15-LO and COX-2 expression was performed on each group on 0, 7, and 14 days. RESULTS: 15-LO expression of mucociliated epithelium was noted in ciliated cells and basal cells, but was not found in goblet cells and secretory acini. In squamous epithelium, the expression of 15-LO was weaker than that in the mucociliated epithelium, but the expression of COX-2 showed no difference between them. In Western blot analysis, 15-LO expression was significantly higher in RA-sufficient culture than in RA-deficient culture and this expression was time-dependent. COX-2 expression was almost same level in RA-deficient culture, but its expression was significantly higher in RA-sufficient culture on 7 and 14 days than on zero day. CONCLUSION: 15-LO and COX-2 may be related to differentiation and development of nasal epithelial cells. However, it is unclear whether this relationship is direct or indirect effect of 15-LO and COX-2. This question remains to be solved.
