The Morphological Study of the Protein Gene Product (PGP) 9.5-like Immunoreactive Enteric Nervous System in Murine Ileum: Close Relation to Interstitial Cells of Cajal (ICC) in Confocal Laser Scanning Microscopy.
- Author:
In Youb CHANG
1
;
Ho Jin YOU
Author Information
1. Department of Anatomy, Medical College, Chosun University, Gwangju, Korea. iyjang@mail.chosun.ac.kr
- Publication Type:Original Article
- Keywords:
PGP9.5;
c-Kit;
Interstitial cells of Cajal (ICC);
Enteric nervous system;
G-I motility;
Mouse
- MeSH:
Animals;
Antibodies;
Enteric Nervous System*;
Fluorescent Antibody Technique, Indirect;
Ileum*;
Immunohistochemistry;
Interstitial Cells of Cajal*;
Mice;
Microscopy, Confocal*;
Myenteric Plexus;
Nerve Fibers;
Neurons
- From:Korean Journal of Anatomy
2002;35(2):153-159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to investigate the morphology of the enteric nervous system and interstitial cells of Cajal (ICC) in the murine ileum. The PGP9.5-like immunoreactive (PGP9.5-LI) neurons and the c-Kit-like immunoreactive (c-Kit-LI) ICCs were stained by indirect immunofluorescence method and were observed under the confocal laser scanning microscopy. According to three dimensional reconstruction study, it was found that the PGP9.5-LI neurons and the c-Kit-LI ICCs were widely distributed in the intestinal wall : (1) In circular muscle layer, PGP9.5-LI nerve fibers were paralell to circular muscle layer. (2) In the myenteric plexus, the PGP9.5-LI nerve were closely apposed to the adjacent PGP9.5-LI nerve, constituting the networks. (3) In double-labeling immunohistochemistry using anti-PGP9.5 and anti-c-Kit antibodies, the c-Kit-LI networks encircled around the neural strands. The characteristic arrangement of the PGP9.5-LI enteric nervous system and the ICC containing c-Kit-positive cells provide a morphological basis upon the mechanism regulating gastro-intestinal motility and the pathogenesis of gatro-intestinal disorders.
