Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica).
10.4142/jvs.2012.13.3.223
- Author:
Shinji HIYAMA
1
;
Toshie SUGIYAMA
;
Seiji KUSUHARA
;
Takashi UCHIDA
Author Information
1. Department of Oral Biology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan. hiyamas@hiroshima-u.ac.jp
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
estrogen receptor alpha;
estrogen receptor beta;
medullary bone;
osteoblasts;
osteoclasts
- MeSH:
Animals;
Bone Resorption/genetics;
Bone and Bones/chemistry/cytology/*metabolism;
Cells, Cultured;
Coturnix/*metabolism;
Estradiol/*pharmacology;
Estrogen Receptor alpha/genetics/*metabolism;
Estrogen Receptor beta/genetics/*metabolism;
Gene Expression Regulation;
Male;
Osteoblasts/chemistry/cytology/*metabolism;
Osteogenesis/genetics;
RNA, Messenger/metabolism;
Reverse Transcriptase Polymerase Chain Reaction
- From:Journal of Veterinary Science
2012;13(3):223-227
- CountryRepublic of Korea
- Language:English
-
Abstract:
The temporal expression of estrogen receptor (ER)-alpha and ER-beta mRNA was examined in male Japanese quails. Femurs of quails receiving 17beta-estradiol underwent RTPCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER-alpha was already observed on day 0 and increased slightly during bone formation whereas ER-beta was hardly detected throughout this process. After osteoclasts appeared on the medullary bone surface, this type of bone disappeared from the bone marrow cavity (days 7~15). ER-alpha expression simultaneously decreased slightly and ER-beta levels remained very low. These results suggest that estrogen activity mediated by ER-alpha not only affects medullary bone formation but also bone resorption.
