The Change of Telomerase Activity by Cisplatin and 5-FU in Head and Neck Cancer Cell Lines.
- Author:
Soo Geun WANG
1
;
Jin Choon LEE
;
Jun Young KIM
;
Byung Joo LEE
;
Hwan Jung ROH
;
Eui Kyung GOH
;
Kyong Myong CHON
;
Cheol Min KIM
Author Information
1. Department of Otolaryngology, College of Medicine, Pusan National University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Telomerase;
Cisplatin;
Fluorouracil;
Head and neck neoplasms
- MeSH:
Cell Line*;
Cisplatin*;
Drug Therapy;
Fluorouracil*;
Head and Neck Neoplasms*;
Head*;
Humans;
Telomerase*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2004;47(5):448-451
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Telomerase activity appears to be associated with cell immortalisation and malignant progres-sion. Telomerase activity has been found in the majority of human tumours, but not in most somatic cells or tissues. We studied the change in the telomerase activity by cisplatin and 5-fluorouracil (5-FU) in head and neck cancer cell lines. SUBJECTS AND METHODS: Three different human head and neck cancer cell lines (PNUH-12, SNU-899, HEp-2) were studied. Cell cytotoxi-city and change of telomerase activity by 5-FU and cisplatin treatment were assessed by MTT and TRAP assays. RESULTS: The telomerase activity was detected in the head and neck cancer cell lines. After the cisplatin treatment, the telomerase activity was decreased in a concentration-dependent manner. However, after 5-FU treatment, the telomerase activity showed a slight decrease or no change at the concentration similar to that of cisplatin in the inhibition of tumor growth. CONCLUSION: Our results can explain one of the reasons why cisplatin is more effective than 5-FU in the chemotherapy of head and neck cancer.
