Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves receiving lactic acid bacteria and lactose supplements.
10.4142/jvs.2012.13.3.261
- Author:
Laureano S FRIZZO
1
;
Maria V ZBRUN
;
Lorena P SOTO
;
Ezequiel BERTOZZI
;
Gabriel J SEQUEIRA
;
Luis E MARTI
;
Marcelo L SIGNORINI
;
Roberto Rodriguez ARMESTO
;
Marcelo R ROSMINI
Author Information
1. Departamento de Salud Publica Veterinaria, Hospital de Salud Animal, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Kreder 2805, Esperanza C.P. S3080HOF, Santa Fe, Argentina. lfrizzo@fcv.unl.edu.ar
- Publication Type:Original Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
- Keywords:
calves;
Lactobacillus;
lactose;
probiotic;
Salmonella Dublin challenge
- MeSH:
Administration, Oral;
Animals;
Animals, Newborn;
Cattle;
Cattle Diseases/*drug therapy/microbiology/pathology;
Dietary Supplements/*analysis;
Feces/microbiology;
Lactobacillus/metabolism;
Lactose/*metabolism;
Male;
Pediococcus/metabolism;
Probiotics/*therapeutic use;
Salmonella Infections, Animal/*drug therapy/microbiology/pathology;
Salmonella enterica/*drug effects/growth & development;
Tissue Distribution
- From:Journal of Veterinary Science
2012;13(3):261-270
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to evaluate the capacity of a lactic acid bacteria (LAB) inoculum to protect calves with or without lactose supplements against Salmonella Dublin infection by evaluating histopathological lesions and pathogen translocation. Fifteen calves were divided into three groups [control group (C-G), a group inoculated with LAB (LAB-G), and a group inoculated with LAB and given lactose supplements (L-LAB-G)] with five, six, and four animals, respectively. The inoculum, composed of Lactobacillus (L.) casei DSPV 318T, L. salivarius DSPV 315T, and Pediococcus acidilactici DSPV 006T, was administered with milk replacer. The LAB-G and L-LAB-G received a daily dose of 109 CFU/kg body weight of each strain throughout the experiment. Lactose was provided to the L-LAB-G in doses of 100 g/day. Salmonella Dublin (2 x 1010 CFU) was orally administered to all animals on day 11 of the experiment. The microscopic lesion index values in target organs were 83%, 70%, and 64.3% (p < 0.05) for the C-G, LAB-G, and L-LAB-G, respectively. Administration of the probiotic inoculum was not fully effective against infection caused by Salmonella. Although probiotic treatment was unable to delay the arrival of pathogen to target organs, it was evident that the inoculum altered the response of animals against pathogen infection.
