The Effect of Superior Cervical Ganglionectomy on Recovery of Olfaction in Induced Anosmic Mice.
- Author:
Nam Soo LEE
1
;
Byung Guk KIM
;
Jong Min PARK
;
Yong Soo PARK
;
Su Whan KIM
;
Seong Won KIM
;
Seung Kyun LEE
Author Information
1. LeNez ENT Clinic, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Anosmia;
Olfaction;
Olfactory receptor neurons;
Sympathetic nerve block
- MeSH:
Animals;
Autonomic Nerve Block;
Ganglionectomy*;
Immune Sera;
Immunohistochemistry;
Mice*;
Olfaction Disorders;
Olfactory Marker Protein;
Olfactory Mucosa;
Olfactory Receptor Neurons;
Regeneration;
Smell*;
Zinc Sulfate
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2005;48(12):1462-1467
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: This study was undertaken to evaluate the effect of superior cervical ganglionectomy (SCG) on anosmia, which is peripherally induced in the mice. MATERIALS AND METHOD: Three groups of mice (BCF1) were studied: normal control (nasal instillation of saline, n=6); zinc sulfate group (nasal instillation of 64 mM zinc sulfate, n=25); SCG group (superior cervical ganglionectomy after nasal instillation of 64 mM zinc sulfate, n=25). Tissues of olfactory mucosa were obtained at 1, 2, 3, 4 and 7 weeks after instillation of zinc sulfate, and processed for immunohistochemistry using antisera to olfactory marker protein (OMP) to evaluate the olfactory regeneration. RESULTS: No OMP-positive cells were observed in the first two weeks after the instillation of zinc sulfate in both zinc sulfate group and the SCG group. However, the OMP-positive cells appeared first at 3 weeks after the instillation in both groups, and gradually increased in number at 4 and 7 weeks. In the SCG group, the increase of OMP-positive cells was significantly greater than those of the zinc sulfate group. The number of OMP-positive cells in the SCG group at 7 weeks was almost similar to that of the normal control group. CONCLUSION: SCG enhances regeneration of olfactory receptor cells at 3 weeks after injury. It was inferred from the above results that SCG has a significant effect on the regeneration of olfactory receptor cells and we suggest that SCG could be an effective treatment modality for olfactory dysfunction.
