Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR.
10.4142/jvs.2012.13.3.323
- Author:
Pam Dachung LUKA
1
;
Chrisostom AYEBAZIBWE
;
David SHAMAKI
;
Frank Norbert MWIINE
;
Joseph ERUME
Author Information
1. Applied Biotechnology Division, National Veterinary Research Institute, PMB 1, Vom, Plateau State, Nigeria. pamluka08@gmail.com
- Publication Type:Brief Communication
- Keywords:
diagnosis;
goats;
peste des petits ruminants;
sample;
sheep
- MeSH:
Animals;
DNA Primers/analysis;
Eye/virology;
Goat Diseases/blood/*diagnosis/epidemiology/virology;
Goats;
Hair/virology;
Nose/virology;
Nucleoproteins/analysis;
Peste-des-Petits-Ruminants/blood/*diagnosis/epidemiology/virology;
Peste-des-petits-ruminants virus/genetics/*isolation & purification;
Pigmentation;
RNA, Viral/genetics/*isolation & purification;
Reverse Transcriptase Polymerase Chain Reaction/*methods/standards/veterinary;
Sheep;
Sheep Diseases/blood/*diagnosis/epidemiology/virology;
Uganda/epidemiology
- From:Journal of Veterinary Science
2012;13(3):323-325
- CountryRepublic of Korea
- Language:English
-
Abstract:
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
