Growth Behavior of Endothelial Cells According to Electrospun poly(D,L-Lactic-Co-Glycolic Acid) Fiber Diameter as a Tissue Engineering Scaffold.
10.1007/s13770-016-0053-7
- Author:
Young Gwang KO
1
;
Ju Hee PARK
;
Jae Baek LEE
;
Hwan Hee OH
;
Won Ho PARK
;
Donghwan CHO
;
Oh Hyeong KWON
Author Information
1. Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Korea. ohkwon@kumoh.ac.kr
- Publication Type:Original Article
- Keywords:
Poly(D,L-lactic-co-glycolic acid);
Nanofiber;
Electrospinning;
Diameter;
Scaffold
- MeSH:
Cell Proliferation;
Endothelial Cells*;
Eosine Yellowish-(YS);
Epoprostenol;
Hematoxylin;
Microscopy, Atomic Force;
Microscopy, Electron, Scanning;
Nanofibers;
Platelet Activation;
Solvents;
Tissue Engineering*
- From:
Tissue Engineering and Regenerative Medicine
2016;13(4):343-351
- CountryRepublic of Korea
- Language:English
-
Abstract:
Investigating the effect of electrospun fiber diameter on endothelial cell proliferation provides an important guidance for the design of a fabric scaffold. In this study, we prepared biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) fibrous nonwoven mats with different fiber diameters ranged from 200 nm to 5 µm using the electrospinning technique. To control the fiber diameters of PLGA mats, 4 mixture solvents [hexafluoro-2-propanol, 2,2,2,-trifluoroethanol:dimethylformamide (9:1), 2,2,2,-trifluoroethanol:hexafluoro-2-propanol (9:1), chloroform] were used. Average diameters were 200 nm, 600 nm, 1.5 µm, and 5.0 µm, respectively. Stereoscopic structure and spatial characterization of fibrous PLGA mats were analyzed using atomic force microscopy and a porosimeter. The mechanical properties of PLGA mats were analyzed using a universal testing machine. The spreading behavior and infiltration of endothelial cells on PLGA mats were visualized by field emission scanning electron microscopy and hematoxylin and eosin staining. Cell proliferation on different PLGA fibers with different diameters was quantified using the MTT assay. Cells on 200 nm diameter PLGA mats showed rapid attachment and spreading. However, the cells did not penetrate the PLGA mat. Cells cultured on 600 nm and 1.5 µm diameter fibers could infiltrate the pores and cell proliferation was dramatically increased after 14 days. Secreted prostacyclin from endothelial cells on each mat was measured to examine the ability to inhibit platelet activation. This basic study on cell proliferation and fiber diameter with physical characterization provides a foundation for studies examining nonwoven fibrous PLGA mats as a tissue engineering scaffold.
