The Efficacy and Safety of Collagen-I and Hypoxic Conditions in Urine-Derived Stem Cell Ex Vivo Culture.
10.1007/s13770-016-9073-6
- Author:
So Young CHUN
1
;
Hyun Tae KIM
;
Se Yun KWON
;
Jeongshik KIM
;
Bum Soo KIM
;
Eun Sang YOO
;
Tae Gyun KWON
Author Information
1. Bio Medical Research Institute, Kyungpook National University Hospital, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Urine-derived stem cells;
Collagen-I;
Hypoxia;
Culture condition;
Mesenchymal stem cell source
- MeSH:
Aging;
Anoxia;
Cell Adhesion;
Cell Cycle;
Cell Proliferation;
Cell- and Tissue-Based Therapy;
Chromosomal Instability;
Collagen Type I;
Extracellular Matrix;
Gene Expression;
Mesenchymal Stromal Cells;
Osteoblasts;
Oxygen;
S Phase;
Stem Cell Niche;
Stem Cells*;
Transcription Factors
- From:
Tissue Engineering and Regenerative Medicine
2016;13(4):403-415
- CountryRepublic of Korea
- Language:English
-
Abstract:
Upper urinary tract-derived urine stem cells (USCs) are considered a valuable mesenchymal stem cell source for autologous cell therapy. However, the reported culture condition for USCs is not appropriate for large-quantity production, because cells can show limited replicativity, senescence, and undesirable differentiation during cultivation. These drawbacks led us to reconstitute a culture condition that mimics the natural stem cell niche. We selected extracellular matrix protein and oxygen tension to optimize the ex vivo expansion of USCs, and compared cell adhesion, proliferation, gene expression, chromosomal stability, differentiation capacity, immunity and safety. Culture on collagen type I (ColI) supported highly enhanced USC proliferation and retention of stem cell properties. In the oxygen tension analysis (with ColI), 5% O₂ hypoxia showed a higher cell proliferation rate, a greater proportion of cells in the S phase of the cell cycle, and normal stem cell properties compared to those observed in cells cultured under 20% O₂ normoxia. The established reconstituted condition (ColI/hypoxia, USCs(recon)) was compared to the control condition. The expanded USCs(recon) showed highly increased cell proliferation and colony forming ability, maintained transcription factors, chromosomal stability, and multi-lineage differentiation capacity (neuron, osteoblast, and adipocyte) compared to the control. In addition, USCs(recon) retained their immune-privileged potential and non-tumorigenicity with in vivo testing at week 8. Therefore, reconstituted condition allows for expanded uUSC cell preparations that are safe and useful for application in stem cell therapy.
