Effect of Nitric Oxide and Peroxynitrite in Mucociliary Transport Function of Experimental Otitis Media with Effusion.
- Author:
Eun Ju JEON
1
;
Yong Soo PARK
;
Sayong CHAE
;
Sang Won YEO
;
Ki Hong CHANG
;
So Young PARK
Author Information
1. Department of Otolaryngology-HNS, Our Lady of Mercy Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. parkent@dreamwiz.com
- Publication Type:Original Article
- Keywords:
Nitric oxide;
Peroxynitrite;
Mucociliary clearance;
Otitis media with effusion
- MeSH:
Animals;
Ear, Middle;
Edema;
Guinea Pigs;
Injections, Intraperitoneal;
Mucociliary Clearance*;
NG-Nitroarginine Methyl Ester;
Nitric Oxide*;
Otitis Media with Effusion*;
Otitis Media*;
Otitis*;
Peroxynitrous Acid*;
Temporal Bone;
Uric Acid
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2003;46(9):720-726
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Nitric oxide (NO) is generally beneficial but potentially toxic to host. It has been suggested that NO is an important mediator in the pathogenesis of otitis media with effusion. The harmful effect of NO is related with NO-derived intermediates such as peroxynitrite (OONO-). The author investigated the role of NO and peroxynitrite on mucociliary activity in experimental otitis media with effusion. MATERIALS AND METHOD: Otitis media with effusion was induced by injecting lipopolysaccharide (LPS) transtympanically in guinea pigs. In the N(G)-nitro-L-arginine methyl ester (L-NAME) group, L-NAME was additionally injected intratympanically and intramuscularly. Uric acid (UA) group was treated with intraperitoneal injection of UA 3 times. After 24 hours, dye transfer time was measured and temporal bones were taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT). RESULTS: Transfer time of dye was prolonged in LPS group, whereas it was significantly reduced in L-NAME or UA treatment group. Inflammatory cell infiltration and mucosal edema were less in the treatment groups than LPS group, but it was not statistically significant. 3-NT was expressed intensely in subepithelial layer of LPS group, and decreased to mild to moderate degree in the treatment groups. CONCLUSION: LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitritemediated pathways. This study suggests that inhibition of NO synthesis or scavenging of peroxynitrite may have an adjunctive role in the future treatment of otitis media with effusion.
