Expression of Protein Kinase C Isotypes in the Albino Guniea Pig Cochlea.
- Author:
Byung Don LEE
1
;
Je Hwan CHOI
;
Hee Joong KIM
;
Myung Sang YU
;
Jae Wook KIM
;
Jae Hyung LEE
;
Dong Wook KIM
;
Hyuck Soon CHANG
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Soonchunhyang University, College of Medicine, Seoul, Korea. bdlee12@hosp.sch.ac.kr
- Publication Type:Original Article
- Keywords:
Cochlea;
Signal transduction;
Protein kinase C
- MeSH:
Animals;
Cell Proliferation;
Cochlea*;
Cytoplasm;
Endolymph;
Exocytosis;
Gene Expression;
Guinea Pigs;
Hair;
Intercellular Signaling Peptides and Proteins;
Neurotransmitter Agents;
Paraffin;
Protein Kinase C*;
Protein Kinases*;
Reproduction;
Second Messenger Systems;
Signal Transduction;
Spiral Ligament of Cochlea
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2003;46(9):733-736
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Protein kinase plays an important role in transmembrane signalling, which is modulated by cellular transduction by second messengers such as inositol-1, 4, 5-triphosphate (IP3), Diacylglycerol (DG), cAMP, to express biological activity by stimulation of hormones, neurotransmitters, antigens, growth factors. Protein kinase Cs participate in signal transduction of cell, secretion of neurotransmitter, regulation of ion conduction, exocytosis, gene expression and cellular proliferation. Also, it raises slow motility by Na+-H+ exchange (NHE) and Ca2+ channel in the outer hair cells. It may also be involved in mechanical transduction, cellular proliferation and reproduction in supporting cells, and in producing endolymphs using the KCNE1 and Na+/K+-ATPase in the lateral wall of cochlea. MATERIALS AND METHOD: We investigated the immunoreactivities of the PKC (alpha, beta, gamma, delta) via paraffin section and surface preparation of the cochlea of albino guinea pigs. RESULTS: PKC alpha immunoreactivities were shown in the outer and inner hair cell cytoplasm and delta was revealed in the type II fibrocytes and suprastrial cells in the basal turn of the spiral ligament. But beta, gamma were not shown. CONCLUSION: We suggest that PKC alpha may induce the slow motility and depolarization to mediate the ion conductance in the hair cells. Also, PKC delta may participate in the production of endolymph. We thus conclude that PKC alpha and delta play an important role in the cochlear signal transduction.
