Expresion of Collagenase-3 (Matrix Metalloproteinase-13) in human middle ear cholesteatoma and granulation.
- Author:
Janghoon CHI
1
;
Jun Young PARK
;
Jin KIM
;
Young Soo RHO
;
Hyun Joon LIM
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, Korea. jhcent@orgio.net
- Publication Type:Original Article
- Keywords:
Cholesteatoma;
Collagenase
- MeSH:
Cartilage;
Cholesteatoma;
Cholesteatoma, Middle Ear*;
Collagenases;
Coloring Agents;
Ear, Middle*;
Extracellular Matrix;
Humans*;
Matrix Metalloproteinase 13*;
Matrix Metalloproteinases;
Mucous Membrane;
Skin
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2001;44(6):572-575
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION: Middle ear cholesteatoma is notorious for its capacity to absorb surrounding bone, but the exact mechanism of bone destruction is not fully understood until now. Recently, a group of matrix metalloproteinases (MMPs) were found to dissolve extracellular matrices. Among them, collagenase-3 (MMP-13), a newly found collagenase, is thought to be a strong enzyme to dissolve bone and cartilage. So we tried to find out whether Collagenase-3 is found in cholesteatoma and plays some role in bone destruction by cholesteatoma. MATERIALS AND METHOD: We performed immunohistochemical stains on 5 cholesteatomas, 5 middle ear granulations, 5 normal middle ear mucosa and 5 deep meatal skin specimens with anticollagenase-3 antibody and analysed the staining patterns. RESULTS: All the cholesteatomas showed strong immunoreactivity with collagenase-3. Two out of 5 granulations showed somewhat weaker and more disseminated patterns of immunoreactivity, but the rest 3 granulations showed no immunoreactivity. Normal middle ear mucosa and deep meatal skin specimens showed no immunoreactivity at all. CONCLUSIONS: Collagenase-3 may play an active role in the process of bone destruction by cholesteatoma; however, a further study using zymography or blotting method is needed to make this clear.
