Effect of Adrenomedullin on Proliferation and Mitogen-Activated Protein Kinase Activity in Osteosarcoma Cell Lines (MG63, U2OS).
10.4055/jkoa.2008.43.6.775
- Author:
Jung Ryul KIM
1
;
Byung Wan CHOI
;
Jong Hyuk PARK
;
Kyu Hyung KIM
Author Information
1. Department of Orthopaedic Surgery, Chonbuk National University Medical School, Research Institute of Clinical Science, Jeonju, Korea. jrkeem@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Adrenomedullin;
Mitogen-activated protein kinase;
Osteosarcoma cell lines;
MG63;
U2OS
- MeSH:
Adrenomedullin;
Blotting, Western;
Cell Line;
Cell Proliferation;
Flavonoids;
Humans;
Osteosarcoma;
Phosphotransferases;
Protein Kinases;
Signal Transduction
- From:The Journal of the Korean Orthopaedic Association
2008;43(6):775-782
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aims investigated how how human osteosarcoma cell proliferation and the MAP kinases cascade are regulated, in the MG63 and U2OS human osteosarcoma cell lines after stimulating them with adrenomedullin (AM) with particular focus on extracellular signal-regulated kinase 1/2 (ERK 1 and 2) activation. MATERIALS AND METHODS: A cell proliferation assay was used to examined the effects of AM on the osteosarcoma cell lines (MG63 and U2OS). The effects of AM on ERK1/2 were examed by Western blot analysis. The roles of ERK 1/2 in the AM-induced proliferative signaling pathways in the two cell types were examed using PD98059, a selective inhibitor of the mitogen activated protein-ERK kinase (MEK) pathway. RESULTS: The addition of AM to the medium containing the osteosarcoma MG63 and U2OS cells induced proliferation in a dose-dependent manner. AM strongly activated ERK 1/2 mediated cell proliferation signaling, which was prevented using PD98059. CONCLUSION: These results suggest that AM plays an important role in the proliferation of human osteosarcoma MG63 and U2OS cells, and ERK kinase pathway plays a signal transduction role in AM treated human osteosarcoma MG63 and U2OS cell lines.
