A comparison of anti-inflammatory activities of green tea and grapefruit seed extract with those of microencapsulated extracts.
10.4163/kjn.2012.45.5.443
- Author:
Yoon Kyung JUN
1
;
Myung Hwan KIM
;
Pil Nam SEONG
;
Moon Jeong CHANG
Author Information
1. Department of Food & Nutrition, Kookmin University, Seoul 136-702, Korea. cmoon@kookmin.ac.kr
- Publication Type:Original Article
- Keywords:
grapefruit seed extract;
green tea extract;
microencapsulation;
RAW 264.7 macrophages;
anti-inflammation
- MeSH:
Cardiovascular Diseases;
Cell Line;
Citrus paradisi;
Dinoprostone;
Drug Compounding;
Inflammation;
Macrophages;
Nitric Oxide;
Nitric Oxide Synthase Type II;
Polysaccharides;
Prostaglandins E;
Seeds;
Tea;
Thiobarbiturates;
Tumor Necrosis Factor-alpha
- From:The Korean Journal of Nutrition
2012;45(5):443-451
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We compared the effects of grapefruit seed extract (GFSE), green tea extract (GT) and their microencapsulated extract on anti-inflammatory activities in murine RAW 264.7 macrophages cell line. In order to protect the bioactive compounds in the extracts, they were microencapsulated with maltodextrin and H2O. Nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-alpha), inducible nitric oxide synthase (iNOS) protein expression and thiobarbiturate reactive substances (TBARS) were analyzed in LPS activated RAW 264.7 macrophages. The green tea extract at the range of 100-600 microg/mL inhibited NO, PGE2 production and iNOS protein expression without cytotoxicity in a dose-dependent manner. Grapefruit seed extract had strong inhibitory effects on NO and PGE production and iNOS protein expression at the range of 5-20 microg/mL without cytotoxicity. Microencapsulation of green tea extract had further inhibitory effects on NO and PGE2 production and on iNOS protein expression, whereas microencapsulated GFSE did not show any further inhibitory effects on these parameters. Taken together, our results suggest that GSFE might be a promising candidate for preventing inflammation related diseases, such as cardiovascular disease, cancer or diabetes, and the microencapsulation of green tea extract could improve its bioactivity.
